强光照和黑暗条件下刺参体壁的基因表达差异     

张立斌  冯其明  孙丽娜  房燕  徐冬雪  张涛  杨红生 《海洋学报(英文版)》2018,37(5):54-66

参对光照变化非常敏感，研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况，通过“对照”与“黑暗”，“对照”与“强光”和“黑暗”与“强光”的比较，在|log₂ ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明，“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多，而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对，发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。  相似文献   

双酶法制备低黏度辛烯基琥珀酸淀粉钠的工艺条件   总被引：4，自引：0，他引：4  

阳元娥  罗发兴  黄强  谭伟 《广东海洋大学学报》2004,24(4):49-53

对双酶法制备用作微胶囊壁材的低黏度辛烯基琥珀酸淀粉钠的工艺路线与工艺条件进行了研究。正交实验结果表明 ,在酯化淀粉乳质量浓度 2 0 %、每 g淀粉α 淀粉酶的用量为 6SKBU的条件下 ,影响产品性能的各因素中 ,糖化酶的用量对产品的DE值和流度的影响最大 ,各因素的重要性依次是 :糖化酶的用量、糖化酶作用时间、α 淀粉酶保温作用时间。最佳工艺条件是 :α 淀粉酶保温作用时间 4min ,每 g淀粉糖化酶用量 0 .2GAU ,糖化酶作用时间 2 .5h ,此时产品的DE值为 30 ,流度为 80左右  相似文献   

Application of micropolar plasticity to post failure analysis in geomechanics     

Majid T. Manzari 《国际地质力学数值与分析法杂志》2004,28(10):1011-1032

A micropolar elastoplastic model for soils is formulated and a series of finite element analyses are employed to demonstrate the use of a micropolar continuum in overcoming the numerical difficulties encountered in application of finite element method in standard Cauchy–Boltzmann continuum. Three examples of failure analysis involving a deep excavation, shallow foundation, and a retaining wall are presented. In all these cases, it is observed that the length scale introduced in the polar continuum regularizes the incremental boundary value problem and allows the numerical simulation to be continued until a clear collapse mechanism is achieved. The issue of grain size effect is also discussed. Copyright © 2004 John Wiley & Sons, Ltd.  相似文献   

Solution to the rotation of the elastic earth by method of rigid dynamics     

Yoshio Kubo 《Celestial Mechanics and Dynamical Astronomy》1990,50(2):165-187

Hamiltonian mechanics is applied to the problem of the rotation of the elastic Earth. We first show the process for the formulation of the Hamiltonian for rotation of a deformable body and the derivation of the equations of motion from it. Then, based on a simple model of deformation, the solution is given for the period of Euler motion, UT1 and the nutation of the elastic Earth. In particular it is shown that the elasticity of the Earth acts on the nutation so as to decrease the Oppolzer terms of the nutation of the rigid Earth by about 30 per cent. The solution is in good agreement with results which have been obtained by other, different approaches.  相似文献   

Plane Symmetric Domain Wall in Lyra Geometry     

Anirudh Pradhan  I. Aotemshi  G.P. Singh 《Astrophysics and Space Science》2003,288(3):315-325

In this paper general solutions are found for domain walls in Lyra geometry in the plane symmetric spacetime metric given by Taub. Expressions for the energy density and pressure of domain walls are derived in both cases of uniform and time varying displacement field β. It is also shown that the results obtained by Rahaman et al [IJMPD, 10, 735 (2001)] are particular case of our solutions. Finally, the geodesic equations and acceleration of the test particle are discussed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

THERMAL EFFECTS OF BUILDING′S EXTERNAL SURFACES IN CITY——Characteristics of Heat Flux into and out of External Wall Surfaces     

ZHANG Yi-ping  HE Yun-ling    LIU Yu-hong  MA You-xin  LI You-rong  DOU Jun-xia     《中国地理科学(英文版)》2004,(4)

1INTRODUCTIONWiththerecentmodernizationandurbanizationofChi-na,urbanareashavegreatlyincreased, andgiantbuild-ings,especiallythosetallerthan100m,havebeendomi-nantincities,whichhave resultedinthegreatincreaseintheproportionofbuilding'sexternalsurfacetototalurbanarea.Thedifferencesofthermalpropertiesmain-lyinducedbysolarradiationbetweenbuilding'sexter-nalsurfacesareobviousfordifferentexposures,whichcaninevitablyinfluencethedistributionsofairtempera-turenearby,eventheverticaldistributionsofurb…  相似文献   

Reliability assessment of serviceability performance of braced retaining walls using a neural network approach     

A. T. C. Goh  F. H. Kulhawy 《国际地质力学数值与分析法杂志》2005,29(6):627-642

In urban environments, one major concern with deep excavations in soft clay is the potentially large ground deformations in and around the excavation. Excessive movements can damage adjacent buildings and utilities. There are many uncertainties associated with the calculation of the ultimate or serviceability performance of a braced excavation system. These include the variabilities of the loadings, geotechnical soil properties, and engineering and geometrical properties of the wall. A risk‐based approach to serviceability performance failure is necessary to incorporate systematically the uncertainties associated with the various design parameters. This paper demonstrates the use of an integrated neural network–reliability method to assess the risk of serviceability failure through the calculation of the reliability index. By first performing a series of parametric studies using the finite element method and then approximating the non‐linear limit state surface (the boundary separating the safe and ‘failure’ domains) through a neural network model, the reliability index can be determined with the aid of a spreadsheet. Two illustrative examples are presented to show how the serviceability performance for braced excavation problems can be assessed using the reliability index. Copyright © 2005 John Wiley & Sons, Ltd.  相似文献   

Finite Element Modeling of Swelling Soil Pressures Using Joint/Interface Elements     

Ahmet Şenol  Mustafa Aytekin 《Geotechnical and Geological Engineering》2008,26(3):313-321

There could be some discontinuities in a soil media such as layers, earth structures, cracks, and fissures so that estimation of stresses and deformations in these types of soil masses are somewhat different from continuous masses. The discontinuities in a soil mass could be considered as a special link between two blocks. Transmitted swelling pressures affected by the soil properties of the backfill especially at the interface between the backfill and retaining wall. In order to estimate the transmitted swelling pressure distribution behind a retaining wall, using with interface element, a new finite element model and a code (SWELPRES) have been developed. To evaluate the effect of backfill characteristics and interface element from the transmitted lateral swelling pressures, four cases of thickness of backfills with or without interface elements are investigated.  相似文献   

Reduction of hydroelastic responses of a very-long floating structure by a floating oscillating-water-column breakwater system     

D.C. Hong  S.Y. Hong  S.W. Hong 《Ocean Engineering》2006,33(5-6):610-634

The hydroelastic responses of a very-long floating structure (VLFS) placed behind a reverse T-shape freely floating breakwater with a built-in oscillating water column (OWC) chamber are analyzed in two dimensions. The Bernoulli–Euler beam equation is coupled with the equations of rigid and elastic motions of the breakwater and the VLFS. The interaction of waves between the floating rigid breakwater and the elastic VLFS is formulated in a consistent manner. It has been shown numerically that the structural deflections of the VLFS can be reduced significantly by a suitably designed reverse T-shape floating breakwater.  相似文献   

Action of Cnoidal Waves on Vertical Walls     

Qiu Dahong  Zang Jun  Jia Ying Academician of the Chinese Academy of Sciences  Professor  the State Key Laboratory of Coastal  Offshore Engineering 《中国海洋工程》1996,(2)

Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves  相似文献